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Journal: The American Journal of Pathology
Article Title: MDM2
doi: 10.2353/ajpath.2006.051351
Figure Lengend Snippet: A: Representative RT-PCR analysis of total RNA from cultured human VSMCs (HASMC). RNA was amplified in the presence of oligonucleotide primers specific for MR (top panel) and 11β-HSD type 2 (bottom panel). Extracts from human kidney were used as a positive control (P). B: Representative immunoblotting studies demonstrating MR and 11β-HSD type 2 proteins in HASMC. Extracts from human kidney were used as a positive control (P). C and D: Immunocytochemical analysis demonstrated that immunopositive cells for MR appear brown as a result of diaminobenzidine colorimetric reaction in HASMC without ligand stimulation (C) or treated with 10 nmol/L aldosterone (D) (Original magnification, ×400).
Article Snippet: Cell Culture and Characterization A cultured human VSMC cell line, ie,
Techniques: Reverse Transcription Polymerase Chain Reaction, Cell Culture, Amplification, Positive Control, Western Blot